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Enzymatic Activity of Protoporphyrinogen-IX Oxidase from Various Plant Species:Its Sensitivity to Peroxidizing Herbicides
Shizuka ISHIDA, Roswitha MILLER-SULGER, Hitoshi KOHNO, Peter BOGER and Ko WAKABAYASHI
J. Pestic. Sci.
25, 18-23 (2000)
Enzyme activity of protoporphyrinogen-IX oxidase (Protox) prepared from 34 kinds of plant sources, containing 18 monocotyledons and 16 dicotyledons was evaluated. Protox(es) originated in monocotyledonous plants such as Zea mays cv. Anjou and DK212, Lolium perenne and Poa annua exhibited high enzyme activity, and Protox(es) in dicotyledonous plants such as Agrostemma githago and Arabidopsis thaliana showed a little higher enzyme activity than that of the above monocotyledonous plants. Highly active Protox(es) obtained from Zea mays cv. Anjou, Lolium perenne, Agrostemma githago and Arabidopsis thaliana were selected for the Protox inhibitory assay of peroxidizing herbicides. Echinochloa utilis was also selected for control. Protox inhibition by six peroxidizing herbicides such as oxyfluorfen, chlorophthalim, BW-91, pyraflufen-ethyl, DLH-1777 and LS-82556 was investigated using highly active Protox from the above monocotyledons and dicotyledons. As a result, six peroxidizing herbicides exhibited high inhibitory activity to Protox. Pyraflufen-ethyl showed the highest inhibition. The order of Protox inhibitory activity was pyraflufen-ethylDoxyfluorfenDBW-91DchlorophthalimDDLH-1777DLS-82556, in experiment using four Protox(es) such as Zea mays cv. Anjou, Echinochloa utilis, Agrostemma githago and Arabidopsis thaliana except Lolium perenne. Oxyfluorfen exhibited the highest inhibitory activity to Protox of Lolium perenne. On the other hand, DLH-1777 showed a little less activity to Protox of Zea mays than that of other four weeds. DLH-1777 may become a model compound to find out new selective herbicides. Protox from four weeds will be used for peroxidizing herbicides assay, since the Protox showed high sensitivity to structually different peroxidizers.
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